spoIIIL [2019-07-29 11:33:00]

component of the [protein|search|SpoIIIA]-[protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] trans-envelope complex, required for the activation of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]

locus: BSU_24660

pI: 10.58

mw: 9.62 kDa

protein length: 59 aa

gene length: 180 bp

function: activation of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]

product: component of the [protein|search|SpoIIIA]-[protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ] trans-envelope complex

essential: no

synonyms: yqzE

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]

Gene

Coordinates: 2,555,887 2,556,066

Phenotypes of a mutant

• reduced [SW|sporulation] efficiency (14% compared to wild type) [Pubmed|26735940]
• the ''[gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL] [gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]'' double mutant has a severe [SW|sporulation] defect (0.001%) [Pubmed|26735940]

The protein

[SW|Localization]

• forespore [Pubmed|26735940]

Expression and Regulation

Operons

• genes: [gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG]-[gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL]
  description: [Pubmed|26735940,2507524]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507524], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|26735940], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|8196543], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|26735940]

  additional information

  • the ''[SW|comGA]-[SW|comGB]-[SW|comGC]-[SW|comGD]-[SW|comGE]-[SW|comGF]-[SW|comGG]-[protein|search|spoIIIL]'' operon requires [SW|NusA] for expression [http://www.nature.com/articles/nmicrobiol20157 Reference]

• genes: [gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL]
  description: [Pubmed|26735940]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|26735940]

  additional information

  • the ''[SW|comGA]-[SW|comGB]-[SW|comGC]-[SW|comGD]-[SW|comGE]-[SW|comGF]-[SW|comGG]-[protein|search|spoIIIL]'' operon requires [SW|NusA] for expression [http://www.nature.com/articles/nmicrobiol20157 Reference]

Biological materials

Mutant

• MGNA-C472 (yqzE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2470 NBRP B. subtilis, Japan]
• BKE24660 ([gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATCACCTCATCATT, downstream forward: _UP4_TAACCGCAAATAAACGAATA
• BKK24660 ([gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATCACCTCATCATT, downstream forward: _UP4_TAACCGCAAATAAACGAATA

References

• 2507524,23033921,12107147,8196543,26735940